Staining slides?

[Ganoderma]

ok, so i am pretty pathetic when it comes to MAKING slides!

 

 

 

i mean really, really BAD at it!

 

 

i dont get it. I mainly dealing with plant stuff, such as cells, leaves, flowers, pollen etc.

 

it seems i always either let not enough soak in, or too much or other things get dyed that i didnt think were going to soak it up etc....

 

 

anyone here experienced with it???

 

is there a specific order one must follow for colours, like this one first, then this one etc (for multi colour stains)?

 

 

also your favoured procedure would be great

 

i have used up all my dyes this year, so i need to get some more...and this time i dont want to waste them (if ya know a good cheap source that has a good variety, feel free to let me know :rolleyes:)

 

thanks for any help!

[hilim]

what is the purpose of that slides preperation?

what you looking for: cells, tissue, gfp, nucleus or others?

which plant?;)

[hilim]

if you need a good source for biology materials , which you could get all the company for one product you must use that link:

iBIO - The BioDirectory for Life Sciences Products :)

good luck

[Ganoderma]

i can only get up to 1000X so nothing too fancy. cross sections of many plant types, from algae to cacti to ferns to trees.

 

*mostly* wanting to get down to leaf cells, stem cells, get good looks at the xylem/phloem and transport areas, reproductive areas and pollen etc. from your reply, it sounds like it is far more complicated than i thought it was???

 

 

thanks for the link hilim!

 

what is your procedure, or is procedure different on everything? lets just pretend i am mostly using cross sections, for a start.

[hilim]

ok, i am not sure my experience would help you, i was looking on parts from many models plant in vivo to follow gfp protein localization, also i stained the cells and coloured them with some nucleus dye- i dont remember which, but i must say i just cut part from the leaf with scalpel? usually were no problems,

but when i had problems with the microscop the photos were less.

:evil:

[Ganoderma]

i can stain things one colour -ok-, but i want to make cross sections with multi coloured stains...like those ones you buy that have 3 different colours in the slide.

 

this i have failed at miserably.....:doh: