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Where does the amino acid at the acceptor stem of tRNA come from? Where are the amino acids synthesized, if not from the tRNA itself, and how does it make its way to the acceptor stem in the first place; what role specifically does the anticodon pairing play in this? And if it is synthesized from the tRNA, how does the tRNA remain unaltered afterwards, and how specifically does the anticodon pairing effect this?

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