learnin to learn Posted October 20, 2005 Report Share Posted October 20, 2005 I want to perform some tests with bovine DNA, but I do not know how to seperate the DNA from the cells. Also how do I store the cells/ DNA so that I can use them for more tha one experiment? Quote Link to comment Share on other sites More sharing options...
MortenS Posted October 20, 2005 Report Share Posted October 20, 2005 Here is a pretty straightforward procedure you can do in your kitchen, that does not require any expensive equipment. http://gslc.genetics.utah.edu/units/activities/extraction/ Quote Link to comment Share on other sites More sharing options...
learnin to learn Posted October 21, 2005 Author Report Share Posted October 21, 2005 Thanks for your help!!!! do you have any advice on how to seperate cells from other cells... example isolating specific organisms found in pond water from other organisms Quote Link to comment Share on other sites More sharing options...
MortenS Posted October 22, 2005 Report Share Posted October 22, 2005 I am sorry, I have much experience in sorting insects, but not much (close to nothing) in sorting minute water-living micro-organisms. I guess the method will depend on what sort(s) of organism you want extracted from your sample. Here are some guesses: If they differ in size, there is allways filters you can use to separate the different sizes. If they differ in weight, it might also be possible to sort them after weight by using a high velocity centrifuge It will probably not be enough to sort them in different species though. Maybe there exists methods that utilize their biology, so that they sort themselves? For bacteria, you can grow them on different substrates, specifically tailored for the bacteria you are interested in. Quote Link to comment Share on other sites More sharing options...
learnin to learn Posted October 22, 2005 Author Report Share Posted October 22, 2005 Thankyou for your help!!!!!!!! Quote Link to comment Share on other sites More sharing options...
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